What cell produces GM-CSF?
GM-CSF is produced by multiple cell types such as activated T cells, B cells, macrophages, monocytes, mast cells, vascular endothelial cells, and fibroblasts (2).
How is GM-CSF made?
GM-CSF is produced by various cell types including macrophages, mast cells, T cells, fibroblasts and endothelial cells 8, 9, mostly in response to immune activation and cytokines that mediate inflammation.
What cell types produce CSF?
CSF is produced by specialised ependymal cells in the choroid plexus of the ventricles of the brain, and absorbed in the arachnoid granulations. There is about 125 mL of CSF at any one time, and about 500 mL is generated every day.
What does GM-CSF do to dendritic cells?
GM-CSF (Csf-2) is a critical cytokine for the in vitro generation of dendritic cells (DCs) and is thought to control the development of inflammatory DCs and resident CD103+ DCs in some tissues.
What is GM-CSF antibody?
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a myelopoietic growth factor and pro-inflammatory cytokine that plays a central role in a broad range of immune-mediated diseases.
What does GM-CSF do?
Granulocyte macrophage colony-stimulating factor (GM-CSF) is a cytokine that promotes myeloid cell development and maturation, and dendritic cell differentiation and survival in vitro.
Why is G-CSF given?
G-CSF injections are given before you donate your stem cells to boost your white cells and to release stem cells into the bloodstream ready to collect. G-CSF is also given to some patients receiving treatment for cancer.
What is GM-CSF protein?
What produced CSF?
choroid plexus epithelium
CSF is produced mainly by the choroid plexus epithelium and ependymal cells of the ventricles and flows into interconnecting chambers; namely, the cisterns and the subarachnoid spaces.
What is the function of microglia?
Abstract. Microglia cells are the immune cells of the central nervous system and consequently play important roles in brain infections and inflammation. Recent in vivo imaging studies have revealed that in the resting healthy brain, microglia are highly dynamic, moving constantly to actively survey the brain parenchyma …
What is the yield of GM-CSF+ cells?
The GM-CSF− fraction from the flow-through was passed over a second column (except for donor A) to increase purity of the negative fraction. The yield of GM-CSF+ cells was 1.2 ± 0.6% of CD4 T cells (mean ± SD) and purity was controlled by flow cytometry (see below).
What is gm-csf2?
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as CSF2, is an important hematopoietic growth factor and immune modulator. It is produced by a variety of cell types including; T cells, macrophages, natural killer cells, endothelial cells and fibroblasts. Its’ role is to promote neutrophil proliferation and maturation.
Can T cells express GM-CSF and IFN-γ simultaneously?
Although a T cell can express GM-CSF simultaneously with other cytokines such as IFN-γ, a “GM-CSF-only-producing” murine T cell subset was also proposed and associated with enhanced encephalitogenic activity over IL-17 and IFN-γ-producing T cells in EAE .
What are the biological effects of GM-CSF on hematopoietic cells?
The biological effects of GM-CSF are mediated through binding to cell surface receptors, which appear to be widely expressed by hematopoietic cells and also by some non-hematopoietic cells, such as endothelial cells. Receptor expression is characterized by low number (20-200/cell) and high affinity (Kd = 20-100 pM).