What does Cre stand for?
CRE stands for carbapenem-resistant Enterobacterales. Enterobacterales are an order of germs, specifically bacteria. Many different types of Enterobacterales can develop resistance, including Klebsiella pneumoniae and Escherichia coli (E. coli).
What is Cre mouse?
A Cre mouse contains a Cre recombinase transgene under the direction of a tissue-specific promoter; a loxP mouse contains two loxP sites that flank a genomic segment of interest, the “floxed” locus. Typically, Cre and loxP mice are produced by using transgenic technology (Nagy 2000).
What is Cre LOX used for?
The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression. Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression.
Where do loxP sites come from?
The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1. Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes.
What is Dio Cre?
Principle of Cre-DIO (double-floxed inverse open reading frame) system. Without Cre, the GOI and reporter gene are inverted and silenced. In the presence of Cre, first, the transgene will be reoriented, then one of the two lox sites in each pair will be excised.
What Cre precautions?
Wear a gown and gloves when caring for patients with CRE. Perform hand hygiene—use alcohol-based hand rub or wash hands with soap and water before and after contact with patient or their environment. Make sure labs immediately alert clinical and infection prevention staff when CRE are identified.
How does Cre loxP work?
How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized and bound by a Cre protein, forming a dimer.
What is Cre science?
Cre recombinase is a widely used tool in the field of molecular biology. The enzyme’s unique and specific recombination system is exploited to manipulate genes and chromosomes in a huge range of research, such as gene knock out or knock in studies.
How do you get rid of CRE?
High and sometimes excessive use of antibiotics promotes the growth of bacteria like CRE. If you have a bacterial infection, your healthcare provider may treat it with an antibiotic. The medicine will work if you have a nonresistant strain of the bacteria.
Is CRE an Mdro?
MDRO = multidrug-resistant microorganism, VRE = vancomycin-resistant Enterococci spp. MRSA = methicillin-resistant Staphylococcus aureus, CRE = carbapenem-resistant Enterobacteriaceae, CRAB = carbapenem-resistant acinetobacter baumannii, CRPA = carbapenem-resistant pseudomonas aeruginosa.
What is leaky Cre?
11. Inducible Cre can be leaky. Cre is sequestered in the cytoplasm when it is fused to the ligand binding domain of the estrogen receptor, and enters the nucleus when exposed to tamoxifen.
How does the Cre system work?