What method is used for analysis of vitamin C?
Ultraviolet (UV) Spectrophotometry. Direct ultraviolet spectrophotometry is a fast, simple and reliable method for the determination of vitamin C.
Why is DCPIP used in the investigation of Vit C?
2,6-dichlorophenolindophenol (DCPIP) can be used to estimate the concentration of vitamin C in food. DCPIP is blue when dissolved in water, is red in acid conditions, and is reduced by ascorbic acid (vitamin C) to a colourless compound.
What is the titration that used for vitamin C?
One way to determine the amount of vitamin C in food is to use a redox titration. The redox reaction is better than an acid-base titration since there are additional acids in a juice, but few of them interfere with the oxidation of ascorbic acid by iodine.
Why does vitamin C change the colour of DCPIP?
DCPIP is commonly used as an indicator for Vitamin C. If vitamin C, which is a good reducing agent is present , the blue dye, which turns pink in acid conditions and is reduced to a colourless compound by ascorbic acid.
How do you measure vitamin C content?
To calculate the amount of vitamin C in 1 test oz., divide the number of mg in the vitamin C tablet by 16 oz. For example, if you use a 1000 mg vitamin C tablet, 1000/16=62.5 mg of vitamin C in every oz. of solution.
How do you isolate vitamin C?
Vitamin C is water soluble, so all you need do is crush or grind the plant material and then shake it up with water. A common method is to grind the material with some clean sand in a mortar with a pestle. The material can then be filtered or centrifuged to get rid of the debris. The vitamin C will be in the water.
What is DCIP used for?
2,6-Dichlorophenolindophenol (DCPIP, DCIP or DPIP) is a chemical compound used as a redox dye. When oxidized, DCPIP is blue with a maximal absorption at 600 nm; when reduced, DCPIP is colorless. DCPIP can be used to measure the rate of photosynthesis.
What is indophenol used for?
Indophenol is used in hair dyes, lubricants, redox materials, liquid crystal displays, fuel cells and chemical-mechanical polishing. It is an environmental pollutant and is toxic to fish.
What oxidizing agent was used in the determination of vitamin C?
potassium triiodide
The oxidizing agent you will use is potassium triiodide which is made by dissolving iodine in an aqueous solution of potassium iodide.
What happens to vitamin C during iodometric titration?
As the iodine is added during the titration, the ascorbic acid is oxidised to dehydroascorbic acid, while the iodine is reduced to iodide ions. Due to this reaction, the iodine formed is immediately reduced to iodide as long as there is any ascorbic acid present.
How does DPIP change from blue to colorless?
When the dye is oxidized, it is blue. When reduced, however, it turns colorless. Since DPIP replaces NADPH in the light reactions, it will turn from blue to colorless when reduced during photosynthesis. This will allow you to monitor the rate of photosynthesis.
How do you make vitamin C solution with DCPIP?
Use a 1% vitamin C solution to decolourise 1cm³ of 1% DCPIP solution Pipette 1cm³ of 1% blue DCPIP into a conical flask . Fill up a burette with the first type of fruit juice to be used and take a note of the start value. Use the burette to slowly add the fruit juice to the DCPIP drop by drop.
How do you test for vitamin C deficiency with a pipette?
c Pipette 2 cm 3 of vitamin C solution into a test tube. d Using a graduated pipette or a burette, add 1% DCPIP drop by drop to the vitamin C solution. Shake the tube gently after adding each drop.
How do you use DCPIP test?
Pipette 1cm³ of 1% blue DCPIP into a test tube. Use an accurate pipette to add 1-3 drops of the first fruit juice to the same test tube and then shake the mixture 3 times. Continue to add drops and shake the contents of the test tube until the blue colour of the DCPIP disappears. Note down the volume of fruit juice that was used up.
What is the concentration of the DCPIP used in this experiment?
The DCPIP was carefully made to the concentration of 0.1 % . In each instance 2 milliliters of DCPIP was taken. The sum, 2 milliliters of DCPIP, was chosen because it was non excessively much or excessively small an sum for the reactions to be seen clearly, without taking excessively much clip.