What does the POU5F1 gene do?
Background. The POU5F1 gene encodes the octamer-binding transcription factor-4 (Oct4). It is crucial in the regulation of pluripotency during embryonic development and widely used as molecular marker of embryonic stem cells (ESCs).
Is POU5F1 transcription factor?
POU5F1 and POU2 are the two principle members constituting the class V POU domain family of transcription factors, thought to have a conserved role in the regulation of pluripotency in vertebrates as well as germ cell maintenance and neural patterning.
What are SOX2 and OCT4?
The transcription factors OCT4 and SOX2 are required for generating induced pluripotent stem cells (iPSCs) and for maintaining embryonic stem cells (ESCs). OCT4 and SOX2 associate and bind to DNA in different configurations depending on the arrangement of their individual DNA binding elements.
What general type of protein does the Oct4 gene encode?
Octamer-binding transcription factor 4, OCT-4, is a transcription factor protein that is encoded by the Pou5f1 gene and is part of the POU (Pit-Oct-Unc) family.
Is Sox2 a protein?
Sox2 is a member of the Sox family of transcription factors, which have been shown to play key roles in many stages of mammalian development. This protein family shares highly conserved DNA binding domains known as HMG (High-mobility group) box domains containing approximately 80 amino acids.
What does nestin stain for?
Nestin has recently received attention as a marker for detecting newly formed endothelial cells. Nestin is an angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
What is the significance of NANOG?
NANOG protein expression is a biomarker that indicates poor clinical outcome in lung, breast, gastric, colorectal, pancreatic, and ovarian cancer as well as in hepatocellular, oral squamous cell, esophageal, and nasopharyngeal carcinoma.
Where is NANOG found?
NANOG has been described to be expressed in the posterior side of the epiblast at the onset of gastrulation. There, NANOG has been implicated in inhibiting embryonic hematopoiesis by repressing the expression of the transcription factor Tal1.
What is the role of Oct4 in embryonic stem cells?
In the past several years, genome-wide identification of Oct4 target genes and Oct4-centered protein interactomes has been reported, indicating that Oct4 exerts tight control over pluripotency regulator expression and protects embryonic stem cells in an undifferentiated state.
What does POU5F1 stand for?
POU5F1 (POU Class 5 Homeobox 1) is a Protein Coding gene. Diseases associated with POU5F1 include Embryonal Carcinoma and Teratoma. Among its related pathways are Transcriptional Regulatory Network in Embryonic Stem Cell and Mesodermal Commitment Pathway. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding.
What are the GO annotations related to the pou5f1b gene?
Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding . An important paralog of this gene is POU5F1B. Transcription factor that binds to the octamer motif (5′-ATTTGCAT-3′).
What is the function of the Pou gene?
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis.
What do germ cells express POU5F1?
Mouse primordial germ cells (PGCs), from which pluripotent embryonic germ cells (EGCs) are derived, express POU5F1, NANOG, and SOX2. Nanog and Oct4 associate with unique repressor complexes on their target genes to control ES cell fate