What is polymer capillary electrophoresis?
Capillary electrophoresis (CE) is a very efficient tool for separating and characterizing synthetic polymers, copolymers, and polyelectrolytes.
How do Electropherograms work?
In simple terms, an electropherogram enables you to visualise the results of that which happens during a stage of DNA analysis known as electrophoresis (a process enabling the sorting of molecules based on size). The fragments of DNA are fluorescently labelled (using fluorescent primers in PCR).
What is microfluidic capillary electrophoresis?
Microfluidic capillary electrophoresis (MCE) is the novel technique resulted from the CE mininaturization as planar separation and analysis device. Suitable validation parameters with their suitable accepted ranges should be tailored for analysis methods utilizing such unique technique (MCE).
What is the process of capillary electrophoresis?
Capillary electrophoresis (CE) is a separation technique that separates molecules in an electric field according to size and charge. CE is performed in a small glass tube called a capillary that is filled with an electrolyte solution.
What does capillary electrophoresis detect?
Capillary electrophoresis (CE) is the primary methodology used for separating and detecting short tandem repeat (STR) alleles in forensic DNA laboratories worldwide. This chapter examines the general principles and components of injection, separation, and detection of STR alleles using CE.
How does capillary electrophoresis differ from gel electrophoresis?
The key difference between capillary electrophoresis and gel electrophoresis is that gel electrophoresis is performed in a vertical or horizontal plane using a polymer gel of standard pore size whereas capillary electrophoresis is performed in a capillary tube with a polymer liquid or a gel.
How are Electropherograms generated?
An electropherogram is a plot of results from an analysis done by electrophoresis automatic sequencing. An electropherogram shows a sequence of data that is produced by an automated DNA sequencing machine. Electropherograms may be used for deriving results from: genealogical DNA testing.
What is the difference between electropherogram and chromatogram?
a chromatogram is the depiction of the elution of material from a chromatographic method. an electropherogram is a chromatogram where the source of data is electrophoresis (such as a gel scan; it is especially important when you don’t have a gel to look at, like with capillary electrophoresis).
What is electroosmotic flow in capillary electrophoresis?
Electroosmotic flow is observed when an electric field is applied to a solution in a capillary that has fixed charges on its interior wall. Charge is accumulated on the inner surface of a capillary when a buffer solution is placed inside the capillary.
What is microchip electrophoresis?
Definition: Microchip electrophoresis (MCE) is a miniaturized form of capillary electrophoresis. Electrophoresis is a common technique to separate macromolecules such as nucleic acids (DNA, RNA) and proteins.
What is DNA capillary electrophoresis?
Capillary electrophoresis (CE) is an alternative to conventional slab gel electrophoresis for the separation of DNA fragments. CE offers a number of advantages over slab gel separations in terms of speed, resolution, sensitivity, and data handling.
Why is capillary electrophoresis important?
Capillary electrophoresis plays a critical role in the development of DNA analysis technologies. It has been the method of choice for DNA analysis techniques commonly used for sequencing, sizing, and aptamer discovery and affinity studies.
What is free solution capillary electrophoresis (CZE)?
CZE, also known as free solution capillary electrophoresis, is the simplest and the most used separation mode in CE. In CZE, a capillary is entirely filled with a carrier or background electrolyte (BGE), providing the buffering capacity and conducting the electric current.
What is capillary zone electrophoresis?
Capillary zone electrophoresis (CZE) is a highly efficient separation technique based on differences in the electrophoretic mobilities of analytes, which, in turn, depend on the charge-to-size ratios. From: TrAC Trends in Analytical Chemistry, 2011
What are the disadvantages of capillary electrophoresis?
A disadvantage of performing CZE in the presence of EOF is variation in the magnitude of EOF, which can compromise migration time and peak area reproducibility. Electroosmotic flow can be controlled or eliminated by coating the capillary with an adsorbed or covalent neutral material, usually a hydrophilic polymer.
What is electroosmotic flow in silica capillaries?
Uncoated fused silica capillaries exhibit electroosmotic flow toward the cathode, and the magnitude of EOF increases with pH. Capillaries coated with an adsorbed or covalent positively charged coating exhibit reversed electroosmotic flow toward the anode.